Aqueous humor drains from the anterior chamber (front) of the eye, primarily through the trabecular meshwork (TM), and into Schlemm’s canal (SC), and then into collector channels, aqueous veins, the episcleral veins and finally into the general venous system. Episcleral venous pressure (EVP) is an important contributor to intraocular pressure (IOP) but is notoriously difficult to measure accurately and precisely in humans and experimental animals. Results obtained using non-invasive techniques have been varied and imprecise in both humans and experimental animals and results using more invasive techniques in experimental animals have also been inexact. The discrepancies in EVP values have made it very difficult to determine the effects of drugs, such as those used to treat glaucoma, on EVP. As part of our current research, we are devising a method for obtaining accurate, reproducible EVP measurements, using non-invasive and/or minimally invasive techniques in an experimental animal model.